Coding

Part:BBa_K2225000:Design

Designed by: Nora Junker   Group: iGEM17_Aachen   (2017-10-25)


AtNHXS1 for S. cerevisiae (Na+/H+ antiporter)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We performed some changes of the sequence of the AtNHXS1 gene in order to eliminate illegal restriction sides and to optimize the codon-usage for Saccharomyces cerevisiae.


Source

The gene originally comes from arabidopsis thaliana, but here is used a mutant of AtNHX as a basis for this gene called AtNHXS1 to increase the uptake of Na+ into the vacuole.

References